You Searched For: Western Blotting Reagents

Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.

Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.


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Supplier: KPL
Description: LumiGLO Peroxidase Chemiluminescent substrate is an all-purpose, luminol-based substrate designed for the rapid and sensitive detection of peroxidase labeled-conjugates in Western blotting and ELISA

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Catalog Number: (AAJ63209-AK)
Supplier: Thermo Scientific Chemicals
Description: Saponin permeating solution, is used as a cell permeabilizing agent.

Supplier: Advansta
Description: ELISABright is an enhanced and highly sensitive chemiluminescent HRP substrate optimized for ELISA applications. This luminol-based substrate demonstrates a wide linear dynamic range and a superior signal-to-noise ratio. ELISABright allows for enhanced detection of low abundance proteins and reduced consumption of antibodies

Supplier: Alkali Scientific
Description: Achieve sensitive detection in chemiluminescence assays with the BrightStar™ Duration HRP Chemiluminescent Substrate, designed for accurate and efficient protein detection.

Catalog Number: (101396-882)
Supplier: Surmodics
Description: Chemiluminescent substrates provide a higher degree of sensitivity than other substrates to enable lower limits of detection to be achieved in ELISA and membrane assays. The increase in sensitivity requires more vigilance during the optimization process through the proper selection of the dioxetane substrate, antibody/antigen titers and blockers. Surmodics provides multiple 1-component AP chemiluminescent substrates with various intensities and wavelength emissions to allow the assay developer to choose the appropropriate substrate for the application.


Catalog Number: (PI80196)
Supplier: Invitrogen
Description: The Pierce™ ECL Plus Western Blotting Substrate is an acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.

Catalog Number: (PI34012)
Supplier: Invitrogen
Description: The 1-Step™ chloronaphthol solution of 4-chloro-1-naphthol for the chromogenic detection of horseradish peroxidase (HRP) activity in Western blot and tissue staining methods.

Supplier: Vector Laboratories
Description: Vector Laboratories alkaline phosphatase (AP) substrate options include standard substrates and ImmPACT substrates with higher sensitivity. The range of vibrant colors facilitates multiplex staining and improved contrast with pigmented tissues and counterstains.

Catalog Number: (101396-952)
Supplier: Surmodics
Description: Chemiluminescent substrates provide a higher degree of sensitivity than other substrates to enable lower limits of detection to be achieved in ELISA and Western Blot assays. The increase in sensitivity requires more vigilance during the optimization process through the proper selection of the luminol substrate, antibody/antigen titers and blockers. Surmodics provides multiple 2-component HRP chemiluminescent substrates with various intensities to allow the assay developer to choose the appropropriate substrate for the application.


Catalog Number: (100359-200)
Supplier: Surmodics
Description: DAB membrane substrate is supplied as a two component liquid substrate system designed for the rapid detection of peroxidase in membrane applications.


Catalog Number: (PI37574)
Supplier: Invitrogen
Description: Thermo Scientific 1-Step Ultra TMB-Blotting Solution is an enhanced single-component horseradish peroxidase (HRP) substrate for Western blotting and immunohistochemistry.

Catalog Number: (PI23500)
Supplier: Invitrogen
Description: The Pierce™ Far-Western Blot Kit for Biotinylated Proteins provide a nonisotopic approach to far-Western analysis that can be performed either directly in the gel or on the membrane


Catalog Number: (PI20148E)
Supplier: Invitrogen
Description: LightShift poly(dI-dC) can be used as competitor for nonspecific DNA binding proteins.

Catalog Number: (PI34005)
Supplier: Invitrogen
Description: Thermo Scientific Pierce OPD is a water-soluble substrate for horseradish peroxidase (HRP) that produces a yellow-orange product detectable at 492nm by ELISA plate readers.

Supplier: Alkali Scientific
Description: Optimize your chemiluminescent assays with the Femto HRP Chemiluminescent Substrate Kits, providing sensitive detection for various applications.

Catalog Number: (PI20158)
Supplier: Invitrogen
Description: The Thermo Scientific LightShift™ Chemiluminescent RNA EMSA Kit provides a non-radioactive solution for studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA).

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
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