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Product Image-77392-872

Natronomonas pharaonis Recombinant Sensory Rhodopsin-2 (from E. coli)

Catalog Number: (77392-872)
Supplier: 0000042190
Description: Sensory rhodopsin-2 (SR-2) from N. pharaonis is a 7-transmembrane protein that acts as a phototaxis receptor in archaebacteria.
Product Image-77392-876

SARS-CoV-2 Recombinant SARS CoV-2 Spike Protein Nanodisc Complex (from HEK293 cells)

Supplier: 0000042190
Description: The coronavirus, also known as SARS-CoV-2, enters the cell by using its surface SPIKE. SPIKE is processed on the cell's surface by TMPRSS2, a serine protease. It then subsequently binds to ACE2 a cell surface receptor.

Product Image-77392-802

Nanodisc Mouse MSP1E3D1-His POPC

Catalog Number: (77392-802)
Supplier: 0000042190
Description: This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.
Product Image-77392-748

Nanodisc MSP2N2-His POPC

Catalog Number: (77392-748)
Supplier: 0000042190
Description: This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.
Product Image-77392-750

Nanodisc MSP2N2-His DMPG

Catalog Number: (77392-750)
Supplier: 0000042190
Description: This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.
Product Image-77392-828

Nanodisc Rat MSP1E3D1-His POPC

Catalog Number: (77392-828)
Supplier: 0000042190
Description: Membrane scaffold protein. Rat origin.
Product Image-77392-822

Nanodisc Rat MSP1D1-His POPC

Catalog Number: (77392-822)
Supplier: 0000042190
Description: Membrane scaffold protein. Rat origin.
Image Unavailable-77392-368

Decyl (2-(trimethylammonio)ethyl) phosphate ≥99.5%, Crystallization grade

Supplier: 0000042190
Description: Phosphocholines, or Fos-cholines, are a novel class of detergents that is well suited for the solubilization, stabilization, and purification of membrane proteins. For screening of the best-suited detergent for a given application, we offer Fos-cholines with chain lengths from C-9 to C-16.

Image Unavailable-77392-332

n-Dodecyl-β-D-maltoside ≥99.5%, Crystallization grade

Supplier: 0000042190
Description: Mild, non-ionic detergents such as Dodecylmaltoside are well suited for the solubilization, stabilization, and purification of membrane proteins. For screening of the best-suited detergent for a given application, we offer maltosides with chain lengths from C-9 to C-13.

Image Unavailable-77392-348

N-Dodecylphosphocholine ≥99.5%, Crystallization grade

Supplier: 0000042190
Description: Phosphocholines, or Fos-cholines, are a novel class of detergents that is well suited for the solubilization, stabilization, and purification of membrane proteins. For screening of the best-suited detergent for a given application, we offer Fos-cholines with chain lengths from C-9 to C-16.

Product Image-77392-488

PureCube DIBMA Glycerol, Screening Kit, HEPES

Catalog Number: (77392-488)
Supplier: 0000042190
Description: DIBMA stands for diisobutylene maleic acid copolymer, and is an extensively researched polymer. The fundamental properties of DIBMA include the absence of light absorption at a wavelength >250 nm, a crucial factor for protein detection via absorbance, and a high resistance to divalent cations.
Product Image-77392-998

PureCube Compact Cartridge Co-NTA

Supplier: CUBE BIOTECH
Description: The PureCube Co-NTA Compact cartridge is an IMAC chromatography column prepacked with PureCube Co-NTA resin.

Product Image-77393-458

PureCube 100 Co-NTA Agarose

Supplier: CUBE BIOTECH
Description: Our PureCube 100 Co-NTA agarose resins are agarose resin beads with a diameter of 100 µm. They are used for the purification of active His-tagged proteins from cells.

Product Image-77393-194

PureCube Epoxy Activated Agarose

Supplier: CUBE BIOTECH
Description: PureCube Epoxy Agarose resin is your best option to couple biomolecules that carry free-standing amine or thiol groups.

Product Image-77393-248

PureCube Ni-NTA Agarose XL

Supplier: CUBE BIOTECH
Description: Our PureCube Ni-NTA agarose resins are your best option to purify His-tagged protein using FPLC, Batch spin, or column chromatography.

Product Image-77392-558

AASTY 11-55

Supplier: CUBE BIOTECH
Description: AASTYs (Acrylic acid-co-styrenes) - like AASTY 11-55 - are highly alternating copolymers, well-suited for generating native lipid nanodiscs. They are a 2022 novel developed series for membrane protein solubilization & stabilization. AASTY 11-55 is named from its molecular weight and Acrylic Acid : Styrene Ratio. These varying ratios of acrylic acid to styrene contribute to the hydrophilic properties of our AASTYs. In general lighter AASTYs, like 6-45 tend to be more aggressive, while heavier AASTYs, such as 11-55 show higher thermodynamic stability.

The exact composition of AASTY copolymers shows different extraction efficiencies, depending on the lipid composition of the lipid bilayers being formulated into nanodiscs. As AASTY is made by controlled radical polymerization techniques, the dispersity of polymer molecular weight distribution is low, and the molecular weights are controlled. This means that excess AASTY copolymer can be removed by dialysis after nanodisc formation. Based on previous findings on SMA, it is the expectation that AASTY of different molecular weights will display different rates of nanodisc formation, extraction efficacy, and stability of resulting nanodiscs.

Every membrane protein solubilization needs to undergo a screening process before. The characteristic phospholipid environment surrounding the different membrane proteins in question performs differently well with each polymer. To support you in this process we offer a handy Screening Kit for AASTYs to test them all. Additionally, we recommend the two following publications if you would like to get further information: Smith et al. 2020 & Timcenko et al. 2022

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
To process your orders without delay, please provide the required business documentation to purchase this product.

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The original product is no longer available. The replacement shown is available.
This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at 1-800-932-5000.
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