You Searched For: Antibodies

Catalog Number: (RL605-708-125)

Supplier: Rockland Immunochemical

Description: Anti-Goat IgG (H&L) antibody is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer specificity.

Catalog Number: (RL603-709-C37)

Supplier: Rockland Immunochemical

Description: Anti-IgG Donkey Polyclonal Antibody (Texas Red®)

Supplier: Enzo Life Sciences

Description: The Hsp60 of Heliothis viescens belongs to a highly conserved family of molecular chaperones from several species, including plant Hsp60 (known as Rubisco binding protein), GroEL, the E.coli Hsp60, and 65 kDa major antigen of mycobacteria. In eukaryotes, Hsp60 is localized in the mitochondrial matrix, and in plants Hsp60 is localized in the chloroplast. Mitochondria, chloroplasts and bacteria share a common ancestry (>1 billion years), and this coupled with the high degree of homology between the divergent Hsp60s suggests that these proteins perform a primitive but vital function similar to all the different species. The common characteristics shared by the Hsp60s from the divergent species include high abundance; induction with environmental stress such as heat shock; homo-oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of Mg2+ and ATP; ATPase activity; and a role in folding and assembly of oligomeric protein structures. Studies support these similarities, showing expression of the single-ring human mitochondrial homolog Hsp60 with its co-chaperonin Hsp10, in a E. coli strain engineered so that the groE operon remained under strict regulatory control. The findings demonstrate that expression of Hsp60-Hsp10 enabled successful performance of all essential in vivo functions of GroEL and its co-chaperonin, GroES. Consistent with their functions as chaperones, Hsp60 and Hsp10 may act as docking molecules with a passive role in the maturation of caspase processing. Data incidates that recombinant Hsp60 and Hsp10 accelerate the activation of procaspase-3 by cytochrome c and dATP in an ATP-dependent manner. Hsps are intracellular proteins thought to serve protective functions against infection and cellular stress; however, several studies reveal a possible link between members of the Hsp60 and a number of autoimmune diseases, atherosclerosis, and chlamydial disease.

SDS

Catalog Number: (95043-776)

Supplier: Enzo Life Sciences

Description: Glucose-regulated protein 94 (Grp94, gp96), an abundant resident endoplasmic reticulum (ER) lumenal stress protein, belongs to the Hsp90 family of molecular chaperones along with cytosolic Hsp90. Grp94 and such other resident soluble proteins of the ER as the Ca 2+ binding protein subfamily (CaBP, CaBPI, CaBP2 and calreticulin) possess the C-terminal tetrapeptide Lys-Asp-Glu-Leu (KDEL), a sorting signal considered responsible for the retention of these proteins in the pre-Golgi compartments. Stress conditions such as glucose starvation and heat shock which promote protein misfolding or unfolding increase Grp94 expression. In addition to a homeostatic role in protein folding and assembly, Grp94 can function in the intracellular trafficking of peptides from the extracellular space to the MHC class I antigen processing pathway of antigen presentation cells. Grp94 and Hsp90 share high sequence identity and apparently identical adenosine nucleotide dependent modes of regulation, although previous data suggests that Hsp90 and Grp94 may differ in their nucleotide binding properties. The N-terminal domain of eukaryotic Hsp90 proteins contains a conserved adenosine nucleotide binding pocket which also serves as the binding site for the Hsp90 inhibitors geldanamycin and radicicol. However, the molecular basis for adenosine nucleotide-dependent regulation of Grp94 remains unclear. Data supports a ligand dependent regulation of Grp94 function, and suggests a model whereby Grp94 function is regulated through a ligand -dependent conversion of Grp94 from an inactive to an active conformation.

Catalog Number: (101106-530)

Supplier: Enzo Life Sciences

Description: Metabolism of inositol phospholipids by intracellular signaling mediators is fundamental to signal transduction in eukaryotic cells. PI-4,5-P2 (PIP2) can be synthesized by phosphorylation of PI-4-P by type I phosphatidylinositol phosphate kinase (PIP5K I), or phosphorylation of PI-5-P by type II PIPK (PIP4K II). PI-4,5-P2 regulation of cellular calcium levels involves its hydrolysis by Phospholipase C (PLC) to produce inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG), which serve as second messengers in the import of calcium via IP3-sensitive ion channels and in the activation of PKC, respectively.

Catalog Number: (RL607-4304)

Supplier: Rockland Immunochemical

Description: This product has been assayed against 1.0 µg of Hamster IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid])

Catalog Number: (RL605-4115)

Supplier: Rockland Immunochemical

Description: Secondary Rabbit Anti-IgG F(ab')2 Reacts with Goat

Catalog Number: (RL602-4202)

Supplier: Rockland Immunochemical

Description: Secondary Rabbit Anti-IgG (H&L) Reacts with Cat (Feline)

SDS

Catalog Number: (RL608-4502)

Supplier: Rockland Immunochemical

Description: Secondary Rabbit Anti-IgG (H&L) Reacts with Horse (Equine)

SDS

Catalog Number: (RL200-301-A97)

Supplier: Rockland Immunochemical

Description: Anti-AcK Mouse Polyclonal Antibody [clone: 7F8]

Catalog Number: (RL200-345-215)

Supplier: Rockland Immunochemical

Description: The emission spectra for this DyLight® conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Catalog Number: (RL100-401-A02)

Supplier: Rockland Immunochemical

Description: This antibody reacts with human Cul2 by western blot and immunoprecipitation.  The antibody immunoprecipitates in vitro translated product and Protein from cell lysates (using HeLa or NIH-3T3).  An 86.9 kDa band corresponding to human Cul2 is detected.

Catalog Number: (RL100-401-963)

Supplier: Rockland Immunochemical

Description: Anti-FBXO4 Rabbit Polyclonal Antibody

Catalog Number: (RL100-401-B14S)

Supplier: Rockland Immunochemical

Description: This antibody has been tested for use in ELISA and western blotting and is specific for human CE1 (carboxylesterase).  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 66 kDa in size corresponding CE1 b.

Catalog Number: (RL100-601-143)

Supplier: Rockland Immunochemical

Description: This product has been assayed against 1.0 µg of D-Amino Acid Oxidase (Pig Kidney) in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG (H&L) (Goat) and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid])

Catalog Number: (RL200-401-869)

Supplier: Rockland Immunochemical

Description: Anti-NRF1 Rabbit Polyclonal Antibody [clone: 7F8]