4,4′-Dianilino-1,1′-binaphthyl-5,5′-disulfonic acid dipotassium salt ≥98%, crystalline solid fluorescent dye

Supplier: Cayman Chemical Company
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Synonyms: Bis-ANS

21820-1 21820-5 21820-10
76305-448EA 40.48 USD
76305-448 76305-450 76305-452
4,4′-Dianilino-1,1′-binaphthyl-5,5′-disulfonic acid dipotassium salt ≥98%, crystalline solid fluorescent dye
4,4′-Dianilino-1,1′-binaphthyl-5,5′-disulfonic acid dipotassium salt

bis-ANS is a high-affinity non-covalent extrinsic fluorescent dye used to analyze protein conformation. Its predominant interaction with proteins is through its hydrophobic phenyl and naphthyl rings.


  • Emission 523 (free), 496 (bound) nm
  • Excitation 390 (free), 484 (bound) nm
  • λmax 222, 275, 395 nm


bis-ANS has an excitation maxima of 390 nm. It has an emission maximum of 523 nm when free in solution but undergoes a blue shift with an increase in fluorescence intensity when bound to protein; for example, when bound to intestinal fatty acid binding protein (FAPB2) it has emission maxima of 484-496 nm. bis-ANS has been used to label mechanically damaged neurons in acute brain slices. It also potently inhibits microtubule assembly.

Formula: C₃₂H₂₂K₂N₂O₆S₂
MW: 672.85 g/mol
Storage Temperature: Freezer
MDL Number: MFCD00011592
CAS Number: 65664-81-5
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