You Searched For: Agarose and Acrylamide

Agarose and acrylamide are the most commonly used electrophoresis gels for their versatility with buffers and ability to generate reproducible results. Agarose typically runs horizontal tests to resolve large DNA fragments while acrylamide runs vertical separations for shorter nucleic acids. Easy to handle, the gels are chosen based on the high resolution desired and the samples. The concentration or ratio of the agarose and acrylamide will determine the overall working porosity.

Agarose and acrylamide are the most commonly used electrophoresis gels for their versatility with buffers and ability to generate reproducible results. Agarose typically runs horizontal tests to resolve large DNA fragments while acrylamide runs vertical separations for shorter nucleic acids. Easy to handle, the gels are chosen based on the high resolution desired and the samples. The concentration or ratio of the agarose and acrylamide will determine the overall working porosity.


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Supplier: AMPLYUS LLC
Description: All-in-one tablets with pre-measured agarose, TBE, and SeeGreen™ stain.

Supplier: Edvotek
Description: Ideal for student skill practice.

Supplier: Ward's
Description: High performance, general-purpose agarose.

Supplier: IBI Scientific
Description: 3:1 Super-Sieve Agarose is designed to have a larger gel network than IBI Ultra Sieve Agarose and is recommended for use in the separation of DNA fragments smaller than 1500bp

Supplier: Biotium
Description: Ultrapure molecular biology grade agarose pre-coated with GelGreen® Nucleic acid gel stain for convenient and safer preparation of precast gels.

Environmentally Preferable

Supplier: RPI
Description: Molecular biology grade agarose.

Supplier: GeneMate
Description: GeneMate 3:1 Agarose is a molecular screening agarose designed to have a larger gel network than GeneMate Hi-Res and is recommended for use in separating DNA fragments smaller than 1000 bp

Catalog Number: (52857-970)
Supplier: Diversified Biotech
Description: Better sieving than agarose, 1 gram Synergel = 2 grams agarose.High Resolution

Small Business Enterprise


Supplier: Corning
Description: Axygen® Agarose Low Electroendosmosis (LE) is a high quality molecular biology grade agarose suitable for analytical and preparative electrophoresis of nucleic acids.

Environmentally Preferable Product available on GSA Advantage®

Catalog Number: (PAV3115)
Supplier: Promega Corporation
Description: Used for the electrophoretic separation of nucleic acids and proteins.

Supplier: Biotium
Description: Ultrapure molecular biology grade agarose pre-coated with GelRed® Nucleic Acid Gel Stain for convenient and safer preparation of precast gels.

Environmentally Preferable

Supplier: GeneMate
Description: Low melting temperature agaroses result from a derivatization process by organic synthesis, which generates methoxylate groups from the basic agarose structure. This results in agaroses with low melting and gelling temperatures when compared to standard agaroses. Why use a low melting point agarose? The low melting temperature allows for the recovery of nucleic acids below their denaturing temperature, thus preventing damage to the nucleic acid. Low gelling temperature ensures the agarose will be in a liquid state at a temperature range where in-gel manipulations can be performed without prior extraction of the DNA from the gel slice. GeneMate LowMelt has the highest melting and gelling temperatures in this class of low melt agaroses, as well as the highest gel strength.

Supplier: GeneMate
Description: GeneMate LowMelt GQA is a low melting temperature agarose with the highest resolving capacity for large DNA fragments >1000bp, including PCR products. This agarose is Genetic Quality Analyzed (GQA) and carries that certification. This ensures that in-gel applications can be performed in remelted agarose, avoiding difficult DNA extraction steps. GeneMate LowMelt GQA agarose is ideal for digestion by agarose enzymes, making it very easy to recover large DNA fragments suitable for cloning or enzymatic processing.

Catalog Number: (470111-860)
Supplier: Edvotek
Description: Provides clearer DNA bands and more resilient gel (20 g—40 standard gels).


Supplier: Promega Corporation
Description: Used for electrophoretic separation of nucleic acids.
Supplier: Electron Microscopy Sciences
Description: Agarose I™ is standard melting/gelling agarose, suitable for routine nucleic acid analytical/preservative applications

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