Pierce™ Melon™ Gel Monoclonal IgG Purification Kit, Thermo Scientific
Supplier: LIFE TECHNOLOGIES MS
Melon™ Gel Monoclonal IgG Purification Kit is a high-yield, mild purification system for monoclonal antibodies from ascites or hybridoma cell culture supernatant, or for large scale antibody purification from serum.
- Higher recovery and purity
- Effective for subclasses of IgG that bind poorly to Protein A or G
- Regenerable, support can be regenerated and used for up to 6 purifications
- Compatible, amine free buffer does not require removal or neutralization
Unlike typical bind-and-elute affinity methods, Melon Gel Purification is based on the binding of non-IgG proteins from a prepared supernatant or ascites sample, thereby allowing the pure IgG to be recovered in the flow-through (non-bound) fraction. Antibodies are recovered ready for use in assays or subsequent conjugation and other modification steps. There is no need to neutralize or desalt to mitigate the effects of harsh elution buffers. Melon Gel Purification saves time compared to classical Protein A or Protein G methods without compromising the quality of the antibody.
Ordering information: 200 ml Gel up to 1 L Cell Culture Supernatant or 200 mL Ascites Fluid
Delivery information: Kit includes Melon™ Gel support (200 mL); Melon™ Gel Purification Buffer (dry mix to make 100X), (1 L); and Melon™ Gel Regenerant (dry mix to make 5X), (1 L).
Learn more
About VWR
Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...