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Pierce 1-Step™ NBT/BCIP plus Suppressor Substrate Solution, Thermo Scientific

Supplier: LIFE TECHNOLOGIES MS

Pierce 1-Step™ NBT/BCIP plus Suppressor Substrate Solution, Thermo Scientific

Pierce 1-Step™ NBT/BCIP plus Suppressor Substrate Solution, Thermo Scientific

34070

PI34070EA 184.62 USD

PI34070

Pierce 1-Step™ NBT/BCIP plus Suppressor Substrate Solution, Thermo Scientific

Electrophoresis Reagents Western Blotting Reagents

The 1-Step™ NBT and BCIP substrates are powders and ready-made solutions of nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate for chromogenic blot and IHC staining with alkaline phosphatase probes.

Sensitive with low background and minimial assay-to-assay variability
Ready-to-use single component

The combination of NBT and BCIP yields an intense, insoluble black-purple precipitate when reacted with alkaline phosphatase, an enzyme conjugate for antibody probes. Contains 1 mM levamisole to inhibit endogenous phosphatase activity, while yielding an intense dark purple precipitate in the presence of calf intestinal phosphatase. Used for immunoblotting, immunohistochemistry, and in situ hybridization applications.

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