N-Succinimidyl S-Acetylthioglycolate (SATA), Pierce™

Supplier: 0000013509

Synonyms: N-Succinimidyl-S-acetylthioacetate, S-Acetylthioglycolic Acid N-Succinimidyl Ester, SATA, 2,5-Dioxopyrrolidin-1-yl 2-(acetylthio)acetate

26102
PI26102EA 124.06 USD
PI26102
N-Succinimidyl S-Acetylthioglycolate (SATA), Pierce™
SATA (N-Succinimidyl S-Acetylthioglycolate)

SATA (and SATP) are reagents for introducing protected sulfhydryls into proteins, peptides and other molecules. for covalent modification of primary amines and addition of a protected sulphhydryl group.


  • Adds a protected sulfhydryl that can be deprotected by hydroxylamine
  • Allows long-term storage of the sulfhydryl-modified molecule
  • Offers more steric freedom than SATA for the unmasked sulfhydryl group
  • Forms cleavable disulfide bonds with other sulfhydryl-containing molecules
  • Reacts with primary amines (eg. lysine residues proteins) to form stable amide bonds
  • Preserves protein activity with its mild, non-denaturing reaction cdonditions


SATA (and SATP) are the N-hydroxysuccinimide (NHS) esters of S-acetylthioacetic and propionic acid. A stable, covalent amide bond is formed from the reaction of the NHS ester with primary amines. The amine reacts with the NHS ester by nucleophilic attack, with N-hydroxysuccinimide being released as a by-product. Deprotection (deacylation) to generate a sulfhydryl for use in cross-linking and other applications is accomplished using hydroxylamine·HCl.

Formula: C₈H₉NO₅S
MW: 231.22 g/mol
MDL Number: MFCD00036891
CAS Number: 76931-93-6

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