S-Nitrosylated Protein Detection Kit (Biotin Switch), Cayman Chemical

Supplier: Cayman Chemical Company

10006518-1
75816-684EA 447.57 USD
75816-684
S-Nitrosylated Protein Detection Kit (Biotin Switch), Cayman Chemical
Assays Protein Assays

For detection of S-Nitrosylated proteins.


  • A modified ‘Biotin-switch’ assay
  • Direct visualization of S-NO proteins in whole cells or tissues
  • Colorimetric or fluorometric detection


Nitric oxide (NO) is produced by three distinct isoforms of nitric oxide synthase and functions as a key signaling molecule in physiology and pathophysiology. NO transduces its effects by reacting either directly with heme and non-heme centers of proteins or indirectly via further oxidation to various reactive nitrogen species (RNS). Cayman’s S-Nitrosylated Protein Detection Kit (Biotin Switch) employs a modification of the Jaffrey et al. 'Biotin-switch' method to allow for the direct visualization of S-NO proteins in whole cells or tissues, as well as by western blot analysis. Using this method, free SH groups are first blocked (an addition of 125.1 amu per residue) and any S-NO bonds present in the sample are then cleaved. Biotinylation of the newly formed SH groups (an addition of 523.6 amu per residue) provides the basis for visualization using streptavidin-based colorimetric or fluorescence detection.

Order Now


Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Learn more About VWR