Protein Carbonyl Colorimetric Assay Kit, Cayman Chemical Company
Supplier: Cayman Chemical Company
Convenient colorimetric assay for measurement of oxidized proteins.
- Measure protein carbonyl content in plasma, serum, urine, tissue homogenates, and cell lysates
- Assay 24 samples in duplicate
- Plate-based colorimetric measurement (360 – 385 nm)
The carbonyls of protein samples are derivatized using 2,4-dinitrophenylhydrazine. Formation of a Schiff base produces the corresponding hydrazone which can be analyzed spectrophotometrically at 360 – 385 nm. This assay can be used to measure oxidized protein in plasma, serum, cell lysates, and tissue homogenates.
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