SensoLyte® Plus 520 MMP-2 Assay Kit (Fluorimetric and Enhanced Selectivity), AnaSpec Inc.

Supplier: Anaspec Inc

AS-72224

103010-664EA 969 USD

103010-664

SensoLyte® Plus 520 MMP-2 Assay Kit (Fluorimetric and Enhanced Selectivity), AnaSpec Inc.

Assays Enzyme Assays

The SensoLyte® Plus 520 MMP-2 Assay Kit is designed specifically for detecting MMP-2 activity in biological samples, such as culture medium, serum, plasma, synovial fluid, and tissue homogenate, which may contain multiple MMPs. A monoclonal anti-human-MMP-2 antibody is employed to pull down MMP-2 from the mixture first. MMP-2 activity is then quantified by a 5-FAM/QXL® 520 fluorescence resonance energy transfer (FRET) peptide. The fluorescence signal is monitored at Ex/Em=490 nm/520 nm upon MMP-2-induced cleavage of the FRET substrate. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds. Ample materials are provided to perform 96 assays in a 96-well format.

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