SensoLyte® Rh110 Factor Xa Assay Kit (Fluorimetric), AnaSpec Inc.

Supplier: Anaspec Inc

AS-72207

103010-622EA 884.23 USD

103010-622

SensoLyte® Rh110 Factor Xa Assay Kit (Fluorimetric), AnaSpec Inc.

Assays Enzyme Assays

Factor Xa (FXa) is a serine endopeptidase composed of two disulfide-linked subunits

The SensoLyte® Rh110 Factor Xa Assay Kit provides a convenient assay for screening of FXa inhibitors or continuous assay of enzyme activity using a fluorogenic substrate. Upon FXa protease cleavage, this substrate generates the Rh110 (rhodamine 110) fluorophore which has a bright green fluorescence that can be detected at excitation/emission=490 nm/520 nm. The longer-wavelength spectra and higher extinction coefficient of the Rh110 provide greater sensitivity and less interference from other reaction components.

FXa leads to blood clot formation by converting prothrombin to thrombin through the prothrombinase complex. FXa is generated from zymogen Factor X via the intrinsic and extrinsic pathways and is the rate-limiting step in the propagation of thrombin generation. In the presence of Ca²⁺ ions, FXa forms prothrombinase with factor Va on the phospholipid membrane of the activated platelets. FXa has emerged as an attractive target for drug discovery for thromboembolic diseases.

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