Bacteria Recombinant Streptavidin (from E. coli)

Supplier: Anaspec Inc

AS-72177-100 AS-72177-1000 AS-72177-5 AS-72177-500
103010-558EA 2250.77 USD
103010-558 103010-560 103010-562 103010-564
Bacteria Recombinant Streptavidin (from E. coli)
Proteins and Peptides

Streptavidin is a nonglycosylated, tetrameric protein, with each subunit able to bind a single molecule of the vitamin biotin. Streptavidin-biotin bond is the strongest known non-covalent interaction with Kd ~10-15 M. Because streptavidin lacks any carbohydrate modification and has a near-neutral pI, it has the advantage of much lower nonspecific binding than avidin. Streptavidin is broadly used in various applications such as immunoassays, histochemistry, FISH (Fluorescence In Situ Hybridization), flow cytometry, microarrays and blot analysis.


The recombinant streptavidin was expressed in E. coli. It shows one major band about 56 kDa on SDS-PAGE. The activity of recombinant streptavidin is ~17 units/mg, where one unit is defined as the amount of protein required to bind 1.0 g of D-biotin. The extinction coefficient of streptavidin is E1% 280=32; 1 mg/mL A280 = 3.2.


The recombinant streptavidin was expressed in E. coli. It shows one major band about 56 kDa on SDS-PAGE. The activity of recombinant streptavidin is

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