NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

Supplier: New England Biolabs (NEB)

E7645L E7645S
102969-108EA 2406 USD
102969-108 102969-110
NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®
Nucleic Acid Reagents Next Generation Sequencing Reagents

The ultra II DNA library prep kit for Illumina meets the challenge of constructing high quality libraries from ever-decreasing input quantities.


  • Get more of what you need, with the highest library yields
  • Use to generate high quality libraries from a broad range of input amounts, from 500 pg to 1 µg
  • Prepare libraries from all your samples, including GC-rich DNA and FFPE DNA samples
  • Improve yields and quality for target enrichment applications
  • Save time with streamlined workflows, reduced hands-on time and automation compatibility


The reagents for each step in the library preparation workflow have been reformulated to enable high yield preparation of high quality libraries from 500 picograms to 1 microgram of input DNA. This new generation of NEBNext reagents uses a fast, streamlined, automatable workflow and enables use of fewer PCR cycles while also improving GC coverage. The kit is also compatible with PCR-free workflows and is effective with challenging samples such as FFPE DNA.

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