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HOOK™ Amine Reactive Biotin Reagents & Kits for Highly Efficient Protein Labeling, G-Biosciences

Supplier: G-Biosciences
HOOK™ Amine Reactive Biotin Reagents & Kits for Highly Efficient Protein Labeling, G-Biosciences
HOOK™ Amine Reactive Biotin Reagents & Kits for Highly Efficient Protein Labeling, G-Biosciences
HOOK™ Amine Reactive Biotin Reagents & Kits for Highly Efficient Protein Labeling, G-Biosciences
HOOK™ Amine Reactive Biotin Reagents & Kits for Highly Efficient Protein Labeling, G-Biosciences
HOOK™ Amine Reactive Biotin Reagents & Kits for Highly Efficient Protein Labeling, G-Biosciences
HOOK™ Amine Reactive Biotin Reagents & Kits for Highly Efficient Protein Labeling, G-Biosciences
HOOK™ Amine Reactive Biotin Reagents & Kits for Highly Efficient Protein Labeling, G-Biosciences
HOOK™ Amine Reactive Biotin Reagents & Kits for Highly Efficient Protein Labeling, G-Biosciences
HOOK™ Amine Reactive Biotin Reagents & Kits for Highly Efficient Protein Labeling, G-Biosciences
HOOK™ Amine Reactive Biotin Reagents & Kits for Highly Efficient Protein Labeling, G-Biosciences
HOOK™ Amine Reactive Biotin Reagents & Kits for Highly Efficient Protein Labeling, G-Biosciences
HOOK™ Amine Reactive Biotin Reagents & Kits for Highly Efficient Protein Labeling, G-Biosciences
HOOK™ Amine Reactive Biotin Reagents & Kits for Highly Efficient Protein Labeling, G-Biosciences
HOOK™ Amine Reactive Biotin Reagents & Kits for Highly Efficient Protein Labeling, G-Biosciences
HOOK™ Amine Reactive Biotin Reagents & Kits for Highly Efficient Protein Labeling, G-Biosciences
HOOK™ Amine Reactive Biotin Reagents & Kits for Highly Efficient Protein Labeling, G-Biosciences
HOOK™ Amine Reactive Biotin Reagents & Kits for Highly Efficient Protein Labeling, G-Biosciences
HOOK™ Amine Reactive Biotin Reagents & Kits for Highly Efficient Protein Labeling, G-Biosciences
HOOK™ Amine Reactive Biotin Reagents & Kits for Highly Efficient Protein Labeling, G-Biosciences
HOOK™ Amine Reactive Biotin Reagents & Kits for Highly Efficient Protein Labeling, G-Biosciences
HOOK™ Amine Reactive Biotin Reagents & Kits for Highly Efficient Protein Labeling, G-Biosciences
HOOK™ Amine Reactive Biotin Reagents & Kits for Highly Efficient Protein Labeling, G-Biosciences
HOOK™ Amine Reactive Biotin Reagents & Kits for Highly Efficient Protein Labeling, G-Biosciences
HOOK™ Amine Reactive Biotin Reagents & Kits for Highly Efficient Protein Labeling, G-Biosciences
HOOK™ Amine Reactive Biotin Reagents & Kits for Highly Efficient Protein Labeling, G-Biosciences
HOOK™ Amine Reactive Biotin Reagents & Kits for Highly Efficient Protein Labeling, G-Biosciences
BG-03 BG-10 BG-05 BS-07 BS-04 BG-00 BG-06 786-698 786-083 BS-10 BG-01 BS-06 BG-02 BG-08 BG-02 BG-01 BS-03 BS-01 BS-06 BS-05 BG-09 BG-06 786-699 BS-02 BS-07 786-698 BS-01 BS-08 BS-05 BG-08 BS-09 BG-07 786-084 BG-10 BG-03 BG-00 786-700 786-700 BG-09 786-084 BS-03 786-699 BG-04 786-083 BG-04 BS-10 BS-02 BS-09 BG-05 BS-04 BS-08 BG-07
71003-306EA 568.58 USD
71003-306 CA82022-428 89167-918 89167-916 89167-914 CA82022-872 CA71002-908 CA82022-430 CA71002-906 CA71002-904 CA82022-878 CA82022-876 82022-428 CA82022-874 CA89167-918 CA89167-916 CA89167-914 71002-922 71002-920 82022-874 CA82022-884 82022-872 82022-430 CA82022-880 82022-878 CA82022-888 82022-876 CA82022-886 71002-914 CA71002-922 71002-912 CA71002-920 71002-910 82022-886 CA82022-892 82022-884 CA82022-890 71002-918 71002-916 82022-880 CA71003-306 82022-888 CA71002-912 CA71002-910 CA71002-918 71002-908 82022-892 CA71002-916 71002-906 CA71002-914 82022-890 71002-904
HOOK™ Amine Reactive Biotin Reagents & Kits for Highly Efficient Protein Labeling, G-Biosciences
Protein Linking and Labeling Systems
The use of biotin for non-radioactive labeling of proteins and nucleic acids has now become an increasingly popular technique in life science research

G-Biosciences' HOOK™ Amine Reactive Biotin Reagents react with protein primary amines. Amines, lysine ε-amines and N-terminal α-amines, are the most abundant group in protein molecules and represent the most common target for biotinylation. For example, BSA contains 59 primary amines, of which up to 35 are available on the surface of the molecules and can be reacted with amine reactive esters.

The most widely used amine reactive biotinylation reagents are the water insoluble N-hydroxysuccinimide (NHS) esters or the water soluble N-hydroxysulfosuccinimide (sulfo-NHS) esters. The addition of a charged sulfonate (SO³⁻) on the N-hydroxysuccinimide ring of the sulfo-NHS esters results in their solubility in water (~10mM), but are not permeable to plasma membranes. The solubility and impermeability to plasma membranes makes them ideal for studying cell surface proteins as they will only react with the protein molecules on the outer surface of plasma membranes. The reaction of the NHS and sulfo-NHS esters with amines is virtually identical leading to the formation of an amide bond and release of NHS or sulfo-NHS.

G-Biosciences' HOOK™ Amine Reactive Biotin Reagents are available as the reagent alone or as part of a biotin labeling kit. The labeling kits are designed and supplied with all the necessary reagents, equipment and instructions necessary for optimal reaction conditions, efficient labeling, removal of unbound biotin, and quantification of biotin labeling, for the conjugation of biotin to protein primary amine groups. Each kit is supplied with 25 milligrams of the specific HOOK™ Amine Reactive Biotin Reagent, a specific Optimizer Buffer™ for enhanced conjugation, Spin-OUT™ columns for rapid (less than 10 minute) purification of labeled protein, and reagents to determine the amount of biotinylation. The kits are each designed for the coupling of 1 to 10 milligrams protein in 1 milliliter buffer, suitable for 10 couplings.

In addition, several of the more commonly used HOOK™ Biotin reagents are available in G-Biosciences' OneQuant™ format. The OneQuant™ format prevents loss of reagent due to repeated weighing, as each vial contains only 1 to 2 milligrams of the specific HOOK™ Biotin Reagent.

Several factors must be considered when coupling a biotin reagent to a protein to ensure a successful reaction. The primary consideration is the selection of the biotinylation reagent itself. G-Biosciences offers a wide range of biotin reagents with variations in their reactive groups, spacer arm lengths, solubility, membrane permeability and reversibility. All of these factors must be considered and are dependent on your protein/peptide.

Ordering information: Accessories included.
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