Taq DNA Polymerase, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' recombinant Taq Polymerase is a highly thermostable DNA polymerase isolated from the thermophile, Thermus aquaticus
G-Biosciences' Taq Polymerase is offered in four convenient sizes as well as a 2X Mastermix, each supplied with our 10X PCR buffer. Our Taq Polymerase 2X Mastermix is a ready-to-use mixture of our high quality Taq DNA polymerase, deoxynucleotides and reaction buffer at a 2X concentration. The 2X MasterMix is convenient, fast and reproducible, with reduced number of pipetting steps, lower contamination and pipetting error risk, and high yields of PCR products with minimal optimization. The 2X MasterMix contains all the necessary reagents for successful amplification, except for the DNA template and primers.
UNIT DEFINITION: One unit (U) of Taq polymerase is defined as the amount of enzyme needed to catalyze the incorporation of 10 nanomoles of deoxyribonucleotides into acid-insoluble material in 30 minutes at 70°C using herring sperm DNA as a substrate.
STORAGE BUFFER: 20 mM TrisCl ( pH8.0), 100 mM KCl, 3 mM MgCl₂, 1 mM DTT, 0.1% Nonidet® P-40, 0.1% Tween® 20, 0.2 mg/mL BSA, 50% (v/v) glycerol.
10X PCR BUFFER: 120 mM Tris-HCl (pH 8.8), 500 mM KCl, 1% Triton® X-100, 100 mM Lycine, 25 mM MgSO₄.
The molecular weight of the recombinant protein is 94kD. The Taq polymerase is able to amplify DNA up to 5kb with an elongation velocity of 0.9-1.2kb/min at 70-75°C. The error rate of this Taq polymerase is ~2.2x10¯⁵ nucleotide¯¹ cycle¯¹. Taq DNA polymerase catalyzes the 5’→3’ synthesis of DNA. The enzyme has no detectable 3’→5’ proofreading exonuclease activity and possesses low 5’→3’ exonuclease activity, which results in a 3’-dA overhang on the PCR product.
Ordering information: Accessories available.
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