FlashTaq™ HotStart DNA Polymerase Kit
Supplier: EMPIRICAL BIOSCIENCE
Chemically modified Taq DNA polymerase designed for reducing non-specific DNA amplification due to primer-dimer formation. FlashTaq™ is completely suppressed prior to activation and regains full enzymatic activity in only 2 min at 95 °C. Taq DNA Polymerase gene is isolated from Thermus aquaticus YT1 and expressed in E. coli. FlashTaq™ HotStart DNA Polymerase is supplied in a storage buffer containing: HEPES, KCl, EDTA, DTT, Glycerol, and other stabilizers.
- Storage and handling: Upon arrival store at −20 °C for provided expiration date
10X Reaction (MgCl₂) buffer: Specially optimized reaction buffer containing: Tris-HCl, KCl, and MgCl₂. This Buffer is supplied in a 10X concentration and should be diluted for use.
10X Reaction (No MgCl₂) buffer: Specially optimized reaction buffer containing: Tris-HCl (pH 8.4) and KCl. This Buffer is supplied in a 10X concentration and should be diluted for use.
25 mM MgCl₂: 25 mM magnesium chloride.
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