Reusable plate electrode designed to directly electroporate adherent cells or electroporate cultured cell monolayers on filters. The adherent cell electrode is a reusable electroporation applicator designed to fit into each single well of a 24-well or 12-well plate.
This plate electrode is specially designed to electroporate cultured monolayers of cells on laminin-coated filters, or on 12 mm polycarbonate or polyester transwell filter units (0.4 mm pore size). It can directly electroporate adherent cells such as human corneal keratocytes, Caco-2, dental pulp cells, NC65 and BCEC and MDCK cells.
The adherent cell electrode consists of two parallel plate electrodes, 7×19 mm with 5 mm gap spacing between the plates. Each plate electrode has a 0.3 mm height insulated foot at both ends of the bottom. When the electrode is placed in a dish, a foot minimises the damage on cells. The high voltage cables are connected to a BTX electroporator, and the electrode is brought down onto the filter until it makes contact with the buffer, usually at 1 to 2 mm above the filter. A combination of HV and LV pulses may be used. Adherent cell electrodes are compatible with Gemini X2, ECM 830, ECM 2001+, and Legacy ECM 2001 generators.
