Human DNase I ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human DNase I in serum, plasma, and other biological fluids.
- Higher throughput: Get results in just 90 minutess, with a single wash step
- Detection Range: 4 - 64 ng/ml
- Sensitivity: 0.21 ng/ml
- Sample Type: Serum, plasma or other biological fluids
- Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%
Human DNase I ELISA kit (A314392) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human DNase I in serum, plasma or other biological fluids. An antibody specific for DNase I has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the DNase I present in each sample is bound to the wells by the immobilized antibody. Biotinylated anti-DNase I antibody, which also binds the DNase I present in each sample, and Streptavidin-HRP, which binds the Biotinylated anti-DNase I antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated anti-DNase I antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of DNase I captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of DNase I in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.
Ordering information: This is a ready-to-use ELISA kit that contains: Pre-coated 96 well microplate, standard solution, standard diluent, biotinylated detection antibody, steptavidin-HRP, wash buffer (25X), substrate solution A, substrate solution B, stop solution, plate sealers, foil pouch, and protocol manual.
