Hi-T4 DNA Ligase

Supplier: New England Biolabs
M2622S M2622L M2622L M2622S
76407-352EA 291 USD
76407-352 CA76407-352 76407-354 CA76407-354
Hi-T4 DNA Ligase
Enzymes
A thermotolerant variant of T4 DNA Ligase, Hi-T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5 phosphate and 3 hydroxyl termini in duplex DNA or RNA and is designed to function at higher temperatures than wild type T4 DNA Ligase.

  • Hi-T4 DNA Ligase is an engineered variant of T4 DNA Ligase with improved thermostability
  • Capable of joining blunt end and cohesive end termini
  • Capable of repairing single stranded nicks in duplex DNA and some DNA/RNA hybrids
  • Supplied with both T4 DNA Ligase buffer and StickTogether DNA Ligase buffer

Hi-T4 DNA Ligase will join blunt end and cohesive end termini as well as repair single-stranded nicks in duplex DNA, RNA or DNA/RNA hybrids at temperatures as high as 50 °C.
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