Phosphate Buffered Salts, Dulbecco's Formula, without Magnesium and Calcium, MP Biomedicals

Supplier: MP BIOMEDICALS (FKA ICN BIOMED

091760420 091760422 091760426
IC091760420PK 72.86 USD
IC091760420 IC091760422 IC091760426
Phosphate Buffered Salts, Dulbecco's Formula, without Magnesium and Calcium, MP Biomedicals
Cell Culture Media

Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions: 1. Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance; 2. Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism; 3. Combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism; 4. Provides a buffering system to maintain the medium within the physiological pH range (7.2-7.6)


  • Phosphate Buffered Salts, Dulbecco's Formula (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells.
  • DPBS is used to irrigate, wash, and dilute mammalian cells.
  • Phosphate buffering maintains the pH in the physiological range.
  • Calcium and magnesium facilitate cell binding and clumping.
  • DPBS without these ions can be used to wash and rinse suspended cells.


Inorganic Salts: Potassium Chloride [KCl]: 200.0000 mg/L; Potassium Phosphate Monobasic [KH2PO4]: 200.0000 mg/L; Sodium Chloride [NaCl]: 8000.0000 mg/L; Sodium Phosphate Dibasic [Na2HPO4]: 1150.0000 mg/L


Moisture content: <1.5%


Solubility: Dissolve one packet of powder in 1 L of distilled water. The pH will be in the range of 7.3 - 7.5. If desired, add sodium bicarbonate. The pH can be adjusted as desired using 1N HCl or 1N NaOH. Sterile filter, if desired. Store liquid at 2 - 8 °C.


pH: 7.4 ± 0.1
Osmolality: 279 ± 15 mM/kg
White powder

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