Purified human IgM is suitable for use as a positive control in antibody identification screening techniques and is recommended as an ELISA coating antigen and control.
- Standard in ELISA (100 μg/ml)
- Dot blot assay
- Product is human IgM and buffer salts.
- From human myeloma, highly purified, immunoabsorbed to remove trace amounts of contaminant proteins.
All blood products should be treated as potentially infectious. Source material from which this product was derived was found negative when tested in accordance with current FDA required tests. No known test methods can offer assurance that products derived from human blood will not transmit infectious agents. The total protein is determined using the Biuret procedure, with bovine albumin as the standard. Each vial is overfilled by approximately 10 percent so that a minimum of 20 mg of human hemoglobin is obtained upon reconstitution.
Human hemoglobin is purified from human red blood cells using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography and immunoabsorption. The product is dialyzed into 0.01M sodium phosphate, 0.07M sodium chloride, pH 7.3, filtered through a 0.22 µm filter, vialed and lyophilized. No preservative has been added.
Reconstitution: Reconstitute product with 1 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.
