Super Broth (Large Capsules), MP Biomedicals
Supplier: MP BIOMEDICALS (FKA ICN BIOMED
Superbroth is used for obtaining high yields of lambda phage and phagemids in E. coli.
- 60 g (60 capsules or 12 Large capsules)/l
- Contents/l: 35 g tryptone, 20 g yeast extract, 5 g NaCl
- Molecular biology certified bacterial Growth medium
- Rich Medium for the growth of E. coli.
- high levels of tryptone and yeast extract
Media Preparation: Reagents are tested and chosen for their ability to promote optimum growth of lambda phage and phagemids in E. coli when combined in SuperBroth formulations. Chosen reagents are combined in the proper proportions according to the Superbroth formulation and thoroughly blended for a uniform distribution. A sample of the blended formulation was used to prepare liquid medium by adding 60 g per liter of purified water. The combinations were mixed by hand for about 1 minute to dissolve and autoclaved at 121 °C for 15 minutes. Autoclaving at higher temperatures or for longer periods will result in darkening of the sterile medium, (a darker color does not noticeably affect growth of cells, only the appearance of the sterile medium).
Superbroth is enriched medium with high levels of tryptone and yeast extract. Due to the high levels of peptides, amino acids, vitamins and cofactors high yields of E. coli cells and lambda bacteriophages are obtained.
Recommended use: superbroth is a rich medium for the growth of lambda phage and phagemids in E. coli in liquid medium. Add 60 g per liter of purified water.
Application includes: Bacteria Cell Culture, Gene Expression, Transformation, Microbiological Analysis.
Certifications: Molecular Biology Certified™.
Delivery information: Supplied with 35 g tryptone, 20 g yeast extract, 5 g NaCl.
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