Steady-Glo Luciferase Assay System, Promega
Supplier: Promega Corporation
Steady-Glo Luciferase Assay System is a homogeneous assay with signal half-life of over 5 hours in commonly used cell culture media. Ideal for batch processing.
- Long-Lived Luminescence for Batch Processing in Multiwell Plates
- Stable luminescence signal with 5-hour half-life
- No need to remove culture medium or wash cells prior to adding assay reagent
- Less sensitive to mixing conditions in multiwell plates. Particularly useful in 384-well plates
High-throughput quantitation of firefly (Photinus pyralis) luciferase expression in mammalian cells is commonly performed by batch processing of 96- and 384-well plates. Steady-Glo Luciferase Assay System is designed for this purpose by providing long-lived luminescence when added to cultured cells. The homogeneous assay provides signal half-lives of over 5 hours in commonly used cell culture media without prior sample processing. Throughput rates of several thousand samples per hour may be achieved with high reproducibility under standard laboratory conditions.
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