Access RT-PCR Systems, Promega®
Supplier: Promega Corporation
Access RT-PCR System is designed for the reverse transcription and PCR amplification of a specific target RNA from total RNA or mRNA.
- Convenient RT-PCR in One Tube
- All the reagents you need for single-tube reverse transcription and PCR
- Separate components allow you to control every step of the reaction
The Access RT-PCR System is designed for the reverse transcription (RT) and PCR amplification of a specific target RNA from total RNA or mRNA. This one-tube, two-enzyme system provides sensitive, quick and reproducible analysis of even rare RNAs. The system uses AMV Reverse Transcriptase (AMV RT) from Avian Myeloblastosis Virus for first-strand DNA synthesis and thermostable Tfl DNA polymerase from Thermus flavus for second-strand cDNA synthesis and DNA amplification. The Access RT-PCR System includes an optimized single-buffer system that permits extremely sensitive detection of RNA transcripts without a requirement for buffer additions between the reverse transcription and PCR amplification steps. This simplifies the procedure and reduces the potential for contaminating the samples. In addition, the improved performance of AMV Reverse Transcriptase at elevated temperatures in the AMV/Tfl 5X Reaction Buffer minimizes problems encountered with secondary structures in RNA.
AccessQuick™ RT-PCR system is a master mix system designed to allow easy set up of one-tube RT-PCR reaction. Tfl DNA polymerase, dNTPs, MgCl2, and reaction buffer are all combined into the master mix.
Ordering information: Each Access RT-PCR system comes with AMV reverse transcriptase, Tfl DNA polymerase, AMV/Tfl 5X reaction buffer, MgSO4, dNTP mix, positive control RNA with carrier, upstream control primer, downstream control primer, and nuclease-free water. The largest unit also comes with four vials of access RT-PCR core reagents. Each AccessQuick system comes with master mix, AMV reverse transcriptase, and nuclease-free water.
Caution: Storage temperature -30°C to -10°C
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