5-Fluoroorotic acid monohydrate ≥98%, powder
Supplier: Zymo Research
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Warning
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Synonyms:
5-FOA, 5-Fluoro-2,6-dioxo-1,2,3,6-tetrahydropyrimidine-4-carboxylic acid hydrate, 5-Fluorouracil-6-carboxylic acid monohydrate
Yeast genetic counter-selection agent.
- Available as an ultra-pure powder (
98% purity) or as a solution in DMSO
Using 5-Fluoroorotic Acid (5-FOA) for the counter-selection of yeast is a common genetic screening method. Curing yeast strains of plasmids, plasmid shuffling, allelic replacement, and two-hybrid screens are methods that can employ the use of 5-FOA. Otherwise nontoxic to yeast, 5-FOA is converted to the toxic form (i.e., 5-flurouracil) in strains expressing the functional URA3 gene coding for orotine-5'-monophosphate decarboxylase that is involved in the synthesis of uracil. Yeast strains that are phenotypically Ura+ become Ura- and 5-FOAR after selection.
The question of 5-FOA solubility is often raised by researchers using ultra-pure (
Formula:
C₅H₃FN₂O₄·1H₂O MW: 174.0 g/mol (anhy) Storage Temperature: Freezer |
MDL Number:
MFCD00150658 CAS Number: 220141-70-8 |
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