Tris Buffer (10X), pH 10.0, Electron Microscopy Sciences

Supplier: Electron Microscopy Sciences

Tris buffer chemical structure

Tris buffer chemical structure

64142-11 64142-12

103303-056EA 117.24 USD

103303-056 103305-626

Tris Buffer (10X), pH 10.0, Electron Microscopy Sciences

Buffers Biological Buffers

This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.

1X buffer solution intended for heat-induced antigen retriever in IHC
Please refer to primary antibody protocol

In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC.

Preparation of Working Solution: Dilute this 10X buffer as needed (e.g. 90 mL of deionized or distilled water+10 ml of this buffer) mix well, 1X buffer can be stored at 2 to 8 °C.

The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.

Caution: Store at 2 - 8 °C. Do not freeze.

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