SOC Powder, MP Biomedicals

Supplier: MP Biomedicals

113031012

IC113031012EA 87.44 USD

IC113031012

SOC Powder, MP Biomedicals

Media for Microbiology Dehydrated Media for Microbiology

SOC Medium is a rich media used primarily in the recovery step of Escherichia coli competent cell transformations.

Special medium for incubating cells immediately after transformation
Allows expression of transferred resistance genes before exposing cells to selective conditions
Maximizes the transformation efficiency of competent cells

Reagents are tested and chosen for their ability to promote optimum growth of E. coli when combined in SOC formulations. Chosen reagents are combined in the proper proportions according to the SOC formulation and thoroughly blended for a uniform distribution. A sample of the blended formulation was used to prepare liquid medium by adding 31 g of powder per liter of purified water. The combinations were mixed by hand for about 1 minute to dissolve and autoclaved at 121 °C for 15 minutes. Autoclaving at higher temperatures or for longer periods will result in darkening of the sterile medium, (a darker color does not noticeably affect growth of cells, only the appearance of the sterile medium).

Ordering information: Small quantities of biological buffers have been added for proper pH adjustment.

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