Proteinase K

Supplier: Zymo Research

D3001-2-20 D3001-2-5
76020-322EA 51.9 USD
76020-322 76020-324
Proteinase K
Enzymes

Proteinase K is a stable serine protease with broad substrate specificity and will degrade many proteins in their native conformation, even in the presence of detergents (e.g., SDS).


  • Specifications: Lyophilized enzyme provided with Proteinase K Storage Buffer.
  • Enzyme Commission Number: (EC 3.4.21.64)
  • Source: Engyodontium album pH and Temperature Range: 4.0 to 12.0 (8.0 is optimum), 25 to 65°C.
  • Specific Activity: Over 30 units/mg protein
  • Unit Definition: One unit (U) of enzyme will hydrolyze urea-denatured hemoglobin to produce 1.0 μmole of tyrosine per minute at pH 7.5 at 37°C.


The enzyme is frequently used in molecular biology applications to digest unwanted proteins such as nucleases from DNA and/or RNA preparations from microorganisms, cells, and plants.

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