ProTEV Plus
Supplier: Promega Corporation
ProTEV Plus is an improved 48kDa version of the Nla protease from tobacco etch virus (TEV) that has been engineered to be more stable than native TEV protease for prolonged enzymatic activity.
- Engineered Protease with Greater Stability, Prolonged Activity Over Native TEV Protease
- Active over broad pH (5.5-8.5) and temperature (4-30°C) range
- Cleaves protein fusions with the ENLYFQ(G/S) sequence
- Use with in-solution and on-column digestions
- Supplied at a concentration of 5u/microl
ProTEV Plus is an improved 48kDa version of the Nla protease from tobacco etch virus (TEV) that has been engineered to be more stable than native TEV protease for prolonged enzymatic activity. It is a highly specific proteolytic enzyme that cleaves within a seven-amino-acid sequence (ENLYFQ(G/S)). ProTEV Plus is active over a wide range of pH values (5.5-8.5) and temperatures (4-30 degrees C). It can be used to cleave protein fusions that have been engineered with the above amino acid sequence at the desired cleavage site. The enzyme is compatible for both in-solution and on-column cleavage reactions. ProTEV Plus also contains an HQ tag (analogous to His tag) located at the N-terminus of the protein, which allows it to be immobilized on Ni-based affinity resins and removed from the cleavage reaction.
The enzyme is compatible for both in-solution and on-column cleavage reactions. ProTEV Plus also contains an HQ tag (analogous to His tag) located at the N-terminus of the protein, which allows it to be immobilized on Ni-based affinity resins and removed from the cleavage reaction.
ProTEV Plus is active over a wide range of pH values (5.5 - 8.5) and temperatures (4 to 30 °C). It can be used to cleave protein fusions that have been engineered with the above amino acid sequence at the desired cleavage site.
Learn more
About VWR
Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...