T4 Polynucleotide Kinase, Promega
Supplier: Promega Corporation
T4 PNK catalyzes the transfer of the gamma-phosphate from ATP to the 5'-terminus of polynucleotides or to mononucleotides bearing a 5'-hydroxyl group.
- For labeling in vitro and sequencing
- Transfers the γ-Phosphate from ATP to the 5´ End of Nucleotides
- Can be inactivated by heating at 68°C for 10 minutes
- Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
- Labels the 5´ end of ssDNA, dsDNA and RNA molecules for use as probes or for sequencing or DNA-protein footprinting
- Supplied at a concentration of 5-10u/microl
T4 Polynucleotide Kinase catalyzes the transfer of the gamma-phosphate from ATP to the 5'-terminus of polynucleotides or to mononucleotides bearing a 5'-hydroxyl group. The enzyme, purified from recombinant E. coli, may be used to phosphorylate RNA, DNA and synthetic oligonucleotides prior to subsequent manipulations such as ligation.
Storage buffer: 20 mM Tris-HCl (pH 7,5), 25 mM KCl, 2 mM DTT, 0,1 mM EDTA, 0,1 μM ATP and 50% (v/v) glycerol.
Delivery information: Kit includes T4 Polynucleotide Kinase (5 - 10 µl) and 10X buffer (700 mM Tris-HCl (pH 7,6 at 25 °C), 100 mM MgCl2, 50 mM DTT)
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