Rabbit Pyruvate Kinase (from Muscle), MP Biomedicals

Supplier: MP BIOMEDICALS (FKA ICN BIOMED

0215199905 0215199925 0215199950 0215199980 0215199901
IC15199905EA 211.85 USD
IC15199905 IC15199925 IC15199950 IC15199980 76102-740
Rabbit Pyruvate Kinase (from Muscle), MP Biomedicals
Enzymes

Pyruvate kinase from rabbit muscle is a tetramer consisting of four equal subunits of molecular weight 57 kDa. Pyruvate kinase catalyzes the following reaction: ADP + Phosphoenolpyruvate → Pyruvate + ATP . Both Mg2+ and K+ are required metal cofactors for optimal activity. The enzyme is inhibited by Ca2+. It is a key enzyme in glycogen metabolism.


  • Presentation: White Crystalline Powder
  • Isoelectric Point (pl): 7.6
  • Optimum pH: 7.5
  • Dissolves readily at 5 mg/mL in 0.06M Tris/HCI pH 7.4 to give a clear colorless solution.


Pyruvate kinase is often used as a coupling enzyme in conjunction with lactic dehydrogenase in quantifying ADP and the activity of enzymes that catalyze the formation of ADP.


Pyruvate kinase is highly specific for phosphoenolpyruvate, but can utilize other dinucleotide triphosphates as substrates in place of ATP including GTP, ITP, dATP, UTP, and CTP.


Protein determined by biuret.


That amount of enzyme causing the oxidation of one micromole of NADH per minute at 25 °C and pH 7.4.

Order Now


Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Learn more About VWR