Matrix Metalloproteinase (MMP) Inhibitor Profiling Kit, Fluorometric RED, Enzo Life Sciences
Supplier: Enzo Life Sciences
The MMP inhibitor profiling kit, fluorometric (also known as fluorimetric) RED is a complete assay system designed to examine the specificity of inhibitors against a panel of ten matrix metalloproteinase enzymes, using a quenched fluorogenic substrate OMNIMMP® RED: TQ3-GABA-Pro-Cha-Abu-Smc-His-Ala-Dab(6’-TAMRA)-Ala-Lys-NH2 [TQ3=quencher; GABA=4-aminobutyric acid; Cha=L-cyclohexylalanine; Abu=2-aminobutyric acid; Smc=S-methyl-L-cysteine; Dab=2,4-diaminobutyric acid; 6’-TAMRA=6’-tetramethylrhodamine]. TAMRA fluorescence is thoroughly quenched by the TQ3 group until cleavage by MMPs separates the two moieties.
The assays are performed in a convenient 96-well microplate format. The compound NNGH is also included as a prototypic control inhibitor.
Ordering information: Includes 10 vials (10 MMP enzymes), 1 vial Substrate (OmniMMP™ RED), 1 vial 6'-TAMRA calibration standard, 1 vial control inhibitor (NNGH), 2 bottles (20 ml each) assay buffer, 1 black 96-well microplate, Instructions.
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