Zymolyase-20T is an enzyme preparation from a submerged culture of Arthrobacter luteus which effectively lyses cell walls of viable yeast cells.
- Lyophilized powder prepared by salting out from the culture fluid with ammonium sulfate
- Contains 20,000 units enzyme/g
- Primary enzyme responsible for lysis of viable yeast cells is ß-1,3-glucan laminaripentaohydrolase
- Hydrolyzes glucose polymers at the ß-1,3-glucan linkages releasing laminaripentaose as the principal product
Zymolyase 20T is used to lyse living yeast cell walls to produce protoplast or spheroplast of various strains of yeast cells
The lytic spectrum of this enzyme includes organisms from the following genera: Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloekera, Kluyveromyces, Lipomyces, Metschikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomcopsis, Saccharomycodes, Schwannimomyces and others.
Activators: Dithiothreitol, 2-mercaptoethanol and other sulfohydryl compounds (as cystein); Essential Enzyme: β-1,3-glucan laminaripentaohydrolase; Other Enzymes: β-1,3-glucanase, ca. 1.5 x 106 units/g, Protease, ca. 1.0 x 104 units/g, Mannanase, ca. 1.0 x 106 units/g, Amylase, Xylanase, Phosphatase, trace.
To sterilize use a non-nitrocellulose filter.
It may also be used for glucan hydrolysis.
