PreCR Repair Mix, New England Biolabs
Supplier: New England Biolabs (NEB)
The PreCR Repair Mix is an enzyme cocktail formulated to repair damaged template DNA prior to its use in the polymerase chain reaction (PCR), microarrays, or other DNA technologies.
The PreCR Repair Mix is active on a broad range of DNA damage, including those that block PCR (e.g., apurinic/apyrimidinic sites, thymidine dimers, nicks and gaps) and those that are mutagenic (e.g., deaminated cytosine and 8-oxo-guanine). In addition, it will remove a variety of moieties from the 3´ end of DNA leaving a hydroxyl group. The PreCR Repair Mix will not repair all damage that inhibits/interferes with PCR. It can be used in conjunction with any thermophilic polymerase.
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