Exonuclease V (RecBCD)
Supplier: New England Biolabs (NEB)
M0345L
M0345S
101710-290EA
355
USD
101710-290
101710-292
Exonuclease V (RecBCD)
Enzymes
Exonuclease V, a RecBCD complex from E.coli has several different enzyme activities, including an ATP-dependent single-stranded DNA endonuclease activity, ss- and ds- DNA exonuclease activity.
The hydrolysis in each case is bi-directional (from both the 3´ and 5´ ends) and processive, producing oligonucleotides. All Exonuclease V activities have divalent cation requirements. Mg2+ is required for the exonuclease activity, while Ca2+ inhibits the exonuclease activity and allows double-stranded DNA unwinding (helicase activity) without hydrolysis.
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