Endonuclease VIII

Supplier: New England Biolabs (NEB)

M0299L M0299S
101228-372EA 361 USD
101228-372 101228-374
Endonuclease VIII
Enzymes

Endonuclease VIII from E.coli acts as both an N-glycosylase and an AP-lyase.


The N-glycosylase activity releases damaged pyrimidines from double-stranded DNA, generating an apurinic (AP site). The AP-lyase activity cleaves 3´ and 5´ to the AP site leaving a 5´ phosphate and a 3´ phosphate. Damaged bases recognized and removed by Endonuclease VIII include urea, 5, 6- dihydroxythymine, thymine glycol, 5-hydroxy-5- methylhydanton, uracil glycol, 6-hydroxy-5, 6-dihydrothymine and methyltartronylurea. While Endonuclease VIII is similar to Endonuclease III, Endonuclease VIII has β and δ lyase activity while Endonuclease III has β lyase activity.

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