You Searched For: Vectors, Plasmids and Libraries

In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.

In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.


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Catalog Number: (PAC9380)
Supplier: Promega Corporation
Description: Powerful system for detecting protein:protein interactions in vivo.


Catalog Number: (PAN1031)
Supplier: Promega Corporation
Description: NanoLuc (Nluc) luciferase is a small enzyme (19.1kDa) engineered for optimal performance as a luminescent reporter. The pNL3.1[Nluc/minP] and pNL3.2[NlucP/minP] Vectors offer a minimal promoter for cloning response elements of interest.

Supplier: ReVacc Scientific
Description: This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron CH.1.1. The S has 18-aa cytoplasmic tail truncation for optimal infection.

Supplier: ReVacc Scientific
Description: This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron BA.4/5 (BA.4 and BA.5 variants share the same Spike mutations). The S has 18-aa cytoplasmic tail truncation for optimal infection.

Supplier: ReVacc Scientific
Description: This pseudotyped virus uses recombinant Vesicular Stomatitis Virus (rVSV) to carry GP protein of Marburgvirus - Angola (GenBank: KU978782.1).

Catalog Number: (101227-160)
Supplier: New England Biolabs (NEB)
Description: pTXB1 is an E.coli expression vector in the IMPACT™ Kit.

Small Business Enterprise


Supplier: New England Biolabs (NEB)
Description: pBR322 DNA is a commonly used plasmid cloning vector in E.coli.

Small Business Enterprise

Catalog Number: (PAC9351)
Supplier: Promega Corporation
Description: The CheckMate/Flexi Vector Mammalian Two-Hybrid System provides a means to study suspected interactions between two proteins or domains and can also be used to generate stable cell lines for cell-based assays.

Catalog Number: (PAG8441)
Supplier: Promega Corporation
Description: Vectors designed for expression of N-terminal-tagged HaloTag fusion proteins in mammalian cells. Once expressed, use the HaloTag fusion protein for cell imaging of protein localization or trafficking in conjunction with the fluorescent HaloTag Ligands.

Catalog Number: (PAG8451)
Supplier: Promega Corporation
Description: Vectors designed for expression of N-terminal-tagged HaloTag fusion proteins in mammalian cells. Once expressed, use the HaloTag fusion protein for cell imaging of protein localization or trafficking in conjunction with the fluorescent HaloTag Ligands.


Catalog Number: (PAE5011)
Supplier: Promega Corporation
Description: pGL4.13[luc2/SV40] ,pGL4.53[luc2/PGK] and pGL4.54[luc2/TK] Vectors are used as controls in dual-luciferase assays where Renilla or NanoLuc luciferase is the experimental reporter. Select the appropriate promoter or test all 3 in your experimental system.

Catalog Number: (PAN1411)
Supplier: Promega Corporation
Description: NanoLuc (Nluc) luciferase is a small bright luminescent reporter enzyme (19.1kDa) engineered for optimal performance. Use the pNL3.2.CMV Vector as a negative control in experiments measuring regulated changes in NanoLuc luciferase expression.

Catalog Number: (PAE4131)
Supplier: Promega Corporation
Description: These seven reporter vectors contain response elements (ARE, p53 RE, AT6 RE, MRE, HSE, HRE and XRE) that are important in various stress signaling pathways, the firefly luciferase gene and hygromycin resistance for selection in cells.

Catalog Number: (PAE4001)
Supplier: Promega Corporation
Description: These seven reporter vectors contain response elements (ARE, p53 RE, AT6 RE, MRE, HSE, HRE and XRE) that are important in various stress signaling pathways, the firefly luciferase gene and hygromycin resistance for selection in cells.

SDS


Catalog Number: (PAN1101)
Supplier: Promega Corporation
Description: The pNL1.3[secNluc], pNL3.3[secNluc/minP], pNL2.3[secNluc/Hygro] and pNL1.3.CMV[secNluc/CMV] Vectors offer a secreted small luciferase reporter in various promoter-driven or promoterless configurations for expression in mammalian cells.

Catalog Number: (PAC8491)
Supplier: Promega Corporation
Description: Designed for high-level expression of proteins in mammalian cells from the CMV promoter without a selectable marker


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