You Searched For: Vectors, Plasmids and Libraries

In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.

In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.


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Catalog Number: (PAL5671)
Supplier: Promega Corporation
Description: Designed for use with Wheat Germ extracts and contain sequences from the barley yellow dwarf virus (BYDV), an RNA plant virus, upstream and downstream of the protein coding region of interest.

Catalog Number: (PAE1340)
Supplier: Promega Corporation
Description: pGL4.44[luc2P/AP1 RE/Hygro], pGL4.49[luc2P/TCF-LEF RE/Hygro], pGL4.29[luc2P/CRE/Hygro], pGL4.30[luc2P/NFAT-RE/Hygro], pGL4.32[luc2P/NF-κB-RE/Hygro], pGL4.33[luc2P/SRE/Hygro] and pGL4.34[luc2P/SRF-RE/Hygro] Vectors can explore various signaling pathways.

Catalog Number: (PAE1330)
Supplier: Promega Corporation
Description: The pmirGLO Vector is designed to quantitatively evaluate microRNA (miRNA) activity by the insertion of miRNA target sites downstream or 3' of the firefly luciferase gene (luc2).

Catalog Number: (PAE3661)
Supplier: Promega Corporation
Description: These seven reporter vectors contain response elements (ARE, p53 RE, AT6 RE, MRE, HSE, HRE and XRE) that are important in various stress signaling pathways, the firefly luciferase gene and hygromycin resistance for selection in cells.

Catalog Number: (PAE8491)
Supplier: Promega Corporation
Description: pGL4.44[luc2P/AP1 RE/Hygro], pGL4.49[luc2P/TCF-LEF RE/Hygro], pGL4.29[luc2P/CRE/Hygro], pGL4.30[luc2P/NFAT-RE/Hygro], pGL4.32[luc2P/NF-κB-RE/Hygro], pGL4.33[luc2P/SRE/Hygro] and pGL4.34[luc2P/SRF-RE/Hygro] Vectors can explore various signaling pathways.

Catalog Number: (PAG2821)
Supplier: Promega Corporation
Description: Vectors designed for expression of N-terminal-tagged HaloTag fusion proteins in mammalian cells. Once expressed, use the HaloTag fusion protein for cell imaging of protein localization or trafficking in conjunction with the fluorescent HaloTag Ligands.

Catalog Number: (PAN1321)
Supplier: Promega Corporation
Description: The pFN31 and pFC32 Vectors generate N- or C-terminal fusions of NanoLuc luciferase with your protein of interest. Use pFN31A/K Nluc CMV-Hygro Flexi Vectors for N-terminal fusions and pFC32A/K Nluc CMV-Hygro Flexi Vectors for C-terminal fusions.

Catalog Number: (PAN1491)
Supplier: Promega Corporation
Description: The pNLCoI Vectors comprise a second-generation coincidence reporter vector system that allow expression of both firefly luciferase (luc2) and NanoLuc Luciferase fused to a PEST destabilization domain (NlucP) from the same mRNA transcript.


Catalog Number: (101228-454)
Supplier: New England Biolabs (NEB)
Description: This is the double-stranded, nicked, circular form of ΦX174 DNA (relaxed form)

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Catalog Number: (101227-168)
Supplier: New England Biolabs (NEB)
Description: pTWIN1 is an E.coli expression vector which can be used with the IMPACT™ Kit (NEB #E6901).


Catalog Number: (PAE4121)
Supplier: Promega Corporation
Description: These seven reporter vectors contain response elements (ARE, p53 RE, AT6 RE, MRE, HSE, HRE and XRE) that are important in various stress signaling pathways, the firefly luciferase gene and hygromycin resistance for selection in cells.

Catalog Number: (PAE4141)
Supplier: Promega Corporation
Description: The pGL4.45[luc2P/ISRE/Hygro], pGL4.47[luc2P/SIE/Hygro], pGL4.48[luc2P/SBE/Hygro] and pGL4.52[luc2P/STAT5RE/Hygro] Vectors are optimized for expression in mammalian cells. Use these vectors to explore cytokine signaling pathways.

Catalog Number: (PAE3751)
Supplier: Promega Corporation
Description: These seven reporter vectors contain response elements (ARE, p53 RE, AT6 RE, MRE, HSE, HRE and XRE) that are important in various stress signaling pathways, the firefly luciferase gene and hygromycin resistance for selection in cells.

SDS


Catalog Number: (PAC8451)
Supplier: Promega Corporation
Description: These untagged vectors are used for inducible expression of proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Supplied as vectors used with the Flexi Cloning System.


Catalog Number: (PAC8481)
Supplier: Promega Corporation
Description: Designed for high-level expression of proteins in mammalian cells from the CMV promoter without a selectable marker


Catalog Number: (PAE1081)
Supplier: Promega Corporation
Description: A positive control vector for monitoring transfection efficiencies of mammalian cells. The SV40 early promoter and enhancer drive transcription of the lacZ gene, which encodes the beta-galactosidase enzyme.

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