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Chromatography buffers optimize protein purification and recovery procedures. Eluting from the stationary phase, the solutions change pH levels for a weakened electrostatic force. The salty substances generate and maintain pH levels for proper ion exchange, size exclusion, and affinity. Simply changing the ratio of acid to salt, the chromatography buffer pH level will change accordingly. Designed for various separation techniques, the concentrated liquids or crystalline format options come premade to cut down on prep work time.

Chromatography buffers optimize protein purification and recovery procedures. Eluting from the stationary phase, the solutions change pH levels for a weakened electrostatic force. The salty substances generate and maintain pH levels for proper ion exchange, size exclusion, and affinity. Simply changing the ratio of acid to salt, the chromatography buffer pH level will change accordingly. Designed for various separation techniques, the concentrated liquids or crystalline format options come premade to cut down on prep work time.


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Description: His Buffer Kit contains carefully prepared buffer concentrates for binding, washing, and elution of histidine-tagged proteins according to recommended protocols. Moreover, a method for optimization of imidazole concentration in the binding buffer is described.
Catalog Number: 95016-744
Supplier: Cytiva

Description: Deliver robust, reproducible gradients with the Thermo Scientific pH buffer platform, which is applicable to a wide range of MAbs and ready to use with existing LC columns and systems, without the need to formulate mobile phases.
Catalog Number: 10053-804
Supplier: Thermo Scientific


Description: Triethylammonium bicarbonate buffers are volatile buffer for HPLC.
Catalog Number: BJ17902-100ML
Supplier: Honeywell Research Chemicals


Description: Ammonia buffer solution, pH 10, for complexometry, Mixture, Laboratory Solution, Synonyms: Ammonium chloride / ammonia, color: colourless, form: liquid, Size: 1L
Catalog Number: BJ33582-1L
Supplier: Honeywell Research Chemicals