RFP Western Blot Kit: for RFP Chemiluminescent Western Blotting, Rockland Immunochemicals
Supplier: Rockland Immunochemical
Red Fluorescent Protein (RFP) Chemiluminescent Western Blot kit
- Chemiluminescent Western Blot Kit for RFP contains all of the necessary reagents
- rapid proven protocol
- extremely high signal detection of our luminol chemiluminescent substrate for the detection of recombinant proteins containing wild type Red Fluorescent Protein (DsRed or RFP) and its variants
- This Kit design includes straightforward procedures and color-coding to allow for ease of use. This kit contains sufficient substrate for up to 30 mini blots at 7.5 x 8 cm2 (1,800 cm2) and is stable for at least 1 year when stored as indicated.
Red Fluorescent Protein (RFP) Western Bloting kit allows for the detection of RFP-tagged recombinant proteins present in cell lysates provided by the user. After protein separation by SDS-PAGE and transfer, the membrane is probed with Rockland's optimized Anti-RFP antibody. Detection of the membrane bound antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialized formulation of luminol, an extremely sensitive, non-radioactive substrate that emits light and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems.
Use Rockland Immunochemicals’ Anti-RFP Chemiluminescent Kit for Western Blotting for detection of RFP-tagged recombinant proteins by western blot. This kit is useful for both western blotting and dot blotting methods. Please read the entire product insert prior to use.
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