A complete system for detection of biotin-labeled nucleic acids for various blotting applications including Northern/Southern blots, ribonuclease protection assays (RPAs), electrophoretic mobility shift assays (EMSAs or gel shifts).
- Fast and sensitive
- Versatile kit protocol (included) is applicable to any biotin-based nucleic acid detection system: Northerns, Southerns, EMSA, RPAs and colony lifts
- Kit contains only the critical detection components for those who already have biotin-labeled probes and an optimized hybridization method developed
It is designed for those researchers who already have biotin-labeled probes and an optimized hybridization method developed to allow for quick and sensitive detection results in a wide variety of protocol applications.
The system uses an enhanced luminol substrate for horseradish peroxidase (HRP) with optimized blocking and wash steps that together produce sensitivity equivalent to radioactive (32P) systems and provide a clean, consistent background required for publication-quality data.
Delivery information: Kit contains stabilized Streptavidin-HRP Conjugate (1,5 mL), Chemiluminescent Substrate (Luminol/Enhancer Solution (80 mL) and Stable Peroxide Solution (80 mL), Blocking Buffer (500 mL), 4X Wash Buffer (500mL) and Substrate Equilibration Buffer (500mL).
