Pierce™ 6 Protein Digest, Equimolar, LC-MS grade, Thermo Scientific
Supplier: 0000013509
Pierce™ 6 protein digest standard is a highly validated equimolar protein digest mixture for use as a quality control sample for mass spectrometry (MS) analysis of proteomic samples.
- Positive control sample - optimized and validated as a quality control standard for mass spectrometry applications
- Excellent sequence coverage - on average, greater than 70% sequence coverage of the 6-protein mixture
- Validated peptide quality - digestion procedure optimized for minimal missed cleavages and over-alkylation
- Rigorously tested - high-quality, consistent, 6-protein digest guaranteed and documented via lot-specific certificates of analysis
- Stable - provided in a stable lyophilized format
The Pierce 6 protein digest standard contains an equimolar mixture of highly pure bovine cytochrome c, lysozyme, alcohol dehydrogenase, bovine serum albumin, apo-transferrin, and beta-galactosidase. The proteins are reduced, alkylated (iodoacetic acid), digested (MS-grade trypsin), and desalted (C18) after trypsin digestion to provide a quality LC/MS-grade standard free of intact protein.
The peptide digest provides excellent sequence coverage with minimal over-alkylation and missed cleavages. This digest has been robustly tested for peptide quality, digestion efficiency, and lot-to-lot uniformity and is suitable for LC and LC-MS applications.
Caution: For research use only. Not for use in diagnostic procedures. Storage –5...–30 °C
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