Pierce Y-PER™ Plus Dialyzable, Yeast Protein Extraction Reagent, Thermo Scientific
Supplier: 0000013509
This extraction reagent is formulated to effectively lyse yeast and bacterial cells to extract and solubilize proteins for analysis
- No reducing or chelating agents
- Designed for room temperature storage
- Fast lysis completes after 20 minutes hands-off incubation
- Requires no rigorous mechanical disruption or lengthy enzymatic treatments
This fully dialyzable formulation, allows complete exchange of the extracted protein into any buffer required by downstream techniques. The tris-based buffer has a very low ionic strength and does not include sodium chloride. The reagent is compatible with both BCA and Coomassie plus protein assays.
The reagent is used for Western blots, GST and histidine-tagged purification based applications.
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