Pierce™ Marfey's Reagent, Thermo Scientific
Supplier: 0000013509
Thermo Scientific Pierce Marfey's Reagent (FDAA) reacts with primary amines to enable the quick and easy separation and quantitation of optical isomers of amino acids by reverse-phase chromatography.
- For precolumn derivatization of primary amines for detection by UV absorbance (λmax = 340nm)
- Provides nanomole sensitivity in typical HPLC applications with amino acids
- Reaction products enable chiral separation of D- and L-amino acids
- Following reverse phase HPLC, derivatives have an extinction coefficient of 3×104 M-1 cm-1
- Store product at room temperature
FDAA is 1-fluoro-2-4-dinitrophenyl-5-L-alanine amide, also called Marfey's reagent
FDAA derivatives can be separated with simple linear gradient of triethylamine phosphate/acetonitrile on a Spheri-5 RP-18 HPLC cartridge column. FDAA reduces the need for a chiral column because the D-derivatives are retained on the column, eluting after the L-derivatives. The derivatization is easy to perform and completed in 90 minutes, resulting in derivatives that are stable for at least 48 hours.
FDAA derivatives of D-amino acids exhibit strong intramolecular bonding, which reduces their polarity relative to the corresponding L-amino acid derivatives. Consequently, the D-derivatives are selectively retained on reverse phase columns and elute much later than corresponding L-derivatives.
Caution: For Research Use Only. Not for use in diagnostic procedures.
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