Pierce™ Recombinant Protein G (from E. coli)

Supplier: 0000013509

31499

PI31499EA 275.69 USD

PI31499

Pierce™ Recombinant Protein G (from E. coli)

Proteins and Peptides

Pierce™ Recombinant peroxidase conjugated (HRP-Horseradish Peroxidase) protein G can be used to probe and detect rabbit and human antibodies, especially IgG isotypes, in western blotting, ELISA, IHC and other immunoassay protocols.

Contains two Fc-binding domains per protein
Does not bind human IgM, IgD or IgA
More efficient than protein A for mouse (IgG1), human (IgG3) and rat, goat and cow antibodies
Less efficient than protein A for guinea pig, pig, dog and cat

Protein G is a bacterial cell wall protein isolated from group G Streptococci. DNA sequencing of native protein G identifies two IgG-binding domains and sites for albumin and cell surface binding. The albumin and cell surface binding domains have been eliminated from recombinant protein G to reduce non specific binding and, therefore, can be used to separate IgG from crude samples. Optimal binding occurs at pH 5, although binding is also effective at pH 7,0 to 7,2.

Form: salt-free powder
A280 of 0.1% solution: 1.0
Isoelectric point (pI): 4.5

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