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Pierce™ Protein A, Biotin

Supplier: 0000013509

Pierce™ Protein A, Biotin

Pierce™ Protein A, Biotin

29989

PI29989EA 232.62 USD

PI29989

Pierce™ Protein A, Biotin

Proteins and Peptides

Thermo Scientific Pierce Protein A to probe and detect rabbit and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols.

Contains four Fc-binding domains per protein
Better than Protein G for polyclonal IgG from rabbit, pig, dog and cat serum
Poorer than Protein G for mouse IgG1, human IgG3 and rat, goat and cow antibodies

Protein A is a cell wall component produced by several strains of Staphylococcus aureus that consists of a single polypeptide chain and contains little or no carbohydrate. The Protein A molecule contains four high-affinity binding sites capable of interacting with the Fc region from IgG of several species including human and rabbit. Optimal binding occurs at pH 8.2, although binding is also effective at neutral or physiological conditions (pH 7.0 to 7.6).

Molecular weight: 46,762 (Apparent MW by SDS-PAGE: 42,000)
Form: salt-free powder
A280 of 0.1% solution:

0.137 (theoretical)
Isoelectric point (pI): 5.16 (theoretical)

Caution: For Research Use Only. Not for use in diagnostic procedures.

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