Pierce™ Phosphoprotein Phosphate Estimation Kit, Thermo Scientific

Supplier: 0000013509

23270
PI23270EA 758.46 USD
PI23270
Pierce™ Phosphoprotein Phosphate Estimation Kit, Thermo Scientific
Assays Protein Assays

Pierce™ Phosphoprotein Phosphate Estimation Assay Kit enables classification and identification of proteins as phosphorylated (serine and threonine), as well as semi-quantitative assessment of the phosphorylation level.


  • Specifically measures phosphoserine (p-Ser) and phosphothreonine (p-Thr) only
  • Test tube and 96-well microplate protocols included and require less than 90 minutes to perform
  • Semi-quantitative, calculate moles of phosphate per mole of protein using the included phosvitin standard
  • Format is adaptable to development of specific assays for pure phosphoproteins when previously characterized standards are available


Applications include identification of proteins as containing phosphoserine or phosphothreonine phosphorylations; estimating the amount of pS and pT phosphorylation; developing specific quantitative assays for well-characterised phosphoproteins of interest.


Delivery information: Complete kit includes Ammonium molybdate Solution (25 mL), Malachite green solution (75 mL), Phosvitin positive control (1 mg), and TBS pack (makes 500 mL).

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