Pierce™ Phosphoprotein Phosphate Estimation Kit, Thermo Scientific
Supplier: 0000013509
Pierce™ Phosphoprotein Phosphate Estimation Assay Kit enables classification and identification of proteins as phosphorylated (serine and threonine), as well as semi-quantitative assessment of the phosphorylation level.
- Specifically measures phosphoserine (p-Ser) and phosphothreonine (p-Thr) only
- Test tube and 96-well microplate protocols included and require less than 90 minutes to perform
- Semi-quantitative, calculate moles of phosphate per mole of protein using the included phosvitin standard
- Format is adaptable to development of specific assays for pure phosphoproteins when previously characterized standards are available
Applications include identification of proteins as containing phosphoserine or phosphothreonine phosphorylations; estimating the amount of pS and pT phosphorylation; developing specific quantitative assays for well-characterised phosphoproteins of interest.
Delivery information: Complete kit includes Ammonium molybdate Solution (25 mL), Malachite green solution (75 mL), Phosvitin positive control (1 mg), and TBS pack (makes 500 mL).
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