Sulfo-SANPAH (Sulfosuccinimidyl-6-(4′-azido-2′-nitrophenylamino)hexanoate), Pierce™
Supplier: 0000013509
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Warning
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Thermo Scientific Pierce Sulfo-SANPAH is a hetero-bifunctional crosslinker that contains an amine-reactive N-hydroxysuccinimide (NHS) ester and a photoactivatable nitrophenyl azide.
- Reactive groups: sulfo-NHS ester and nitrophenyl azide
- Reactive towards: amino groups and any nucleophile
- Non-cleavable
- N-Sulfosuccinimidyl-6-(4'-azido-2'-nitrophenylamino) hexanoate
- Optimal photolysis occurs at 320-350 nm; minimizes damage to biomolecules by irradiation
- Water soluble; non-cleavable
NHS esters react efficiently with primary amino groups (-NH2) in pH 7–9 buffers to form stable amide bonds. The reaction results in the release of sulfo-N-hydroxy-succinimide. When exposed to UV light, nitrophenyl azides form a nitrene group that can initiate addition reactions with double bonds, insertion into C-H and N-H sites, or subsequent ring expansion to react with a nucleophile (e.g., primary amines). Sulfo-SANPAH, supplied as a sodium salt, is useful for cell-surface protein crosslinking and possesses charged groups for water solubility to a concentration of 10 mM.
Caution: For Research Use Only. Not for use in diagnostic procedures.
Formula:
C₁₆H₁₇O₉N₆NaS Storage Temperature: Freezer |
CAS Number:
102568-43-4 |
Specification Test Results
Product Line | Pierce™ |
Labeling Method | Chemical Labeling |
Crosslinker Type | Heterobifunctional |
Reactive Moiety | Sulfo-NHS Ester, Aryl Azide |
Spacer | Long |
Form | Solid |
Quantity | 50 mg |
Type | Crosslinker |
Solubility | Water |
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