Sulfo-SANPAH (Sulfosuccinimidyl-6-(4′-azido-2′-nitrophenylamino)hexanoate), Pierce™

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22589
PI22589EA 431.2 USD
PI22589
Sulfo-SANPAH (Sulfosuccinimidyl-6-(4′-azido-2′-nitrophenylamino)hexanoate), Pierce™
Sulfo-SANPAH (Sulfosuccinimidyl-6-(4′-azido-2′-nitrophenylamino)hexanoate)

Thermo Scientific Pierce Sulfo-SANPAH is a hetero-bifunctional crosslinker that contains an amine-reactive N-hydroxysuccinimide (NHS) ester and a photoactivatable nitrophenyl azide.


  • Reactive groups: sulfo-NHS ester and nitrophenyl azide
  • Reactive towards: amino groups and any nucleophile
  • Non-cleavable
  • N-Sulfosuccinimidyl-6-(4'-azido-2'-nitrophenylamino) hexanoate
  • Optimal photolysis occurs at 320-350 nm; minimizes damage to biomolecules by irradiation
  • Water soluble; non-cleavable


NHS esters react efficiently with primary amino groups (-NH2) in pH 7–9 buffers to form stable amide bonds. The reaction results in the release of sulfo-N-hydroxy-succinimide. When exposed to UV light, nitrophenyl azides form a nitrene group that can initiate addition reactions with double bonds, insertion into C-H and N-H sites, or subsequent ring expansion to react with a nucleophile (e.g., primary amines). Sulfo-SANPAH, supplied as a sodium salt, is useful for cell-surface protein crosslinking and possesses charged groups for water solubility to a concentration of 10 mM.


Caution: For Research Use Only. Not for use in diagnostic procedures.

Formula: C₁₆H₁₇O₉N₆NaS
Storage Temperature: Freezer
CAS Number: 102568-43-4

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Specification Test Results

Product Line Pierce™
Labeling Method Chemical Labeling
Crosslinker Type Heterobifunctional
Reactive Moiety Sulfo-NHS Ester, Aryl Azide
Spacer Long
Form Solid
Quantity 50 mg
Type Crosslinker
Solubility Water


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