Sulfo-EMCS (N-(ε-maleimidocaproyloxy) sulfosuccinimide ester), Pierce™
Supplier: 0000013509
Thermo Scientific Pierce Sulfo-EMCS is a water-soluble amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length spacer arm (9.4 angstroms).
- Reactive groups: sulfo-NHS ester and maleimide
- Reactive towards: amino and sulfhydryl groups
- Sulfo-NHS ester end couples with primary amines at pH 7-9 to form stable amide bonds
- Maleimide reacts with -SH groups at pH 6.5-7.5, forming stable thioether linkages
- Non-cleavable
- Water-soluble (compare to EMCS)
- Aliphatic spacer offers low potential for eliciting an immune response
Sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (Sulfo-SMCC) is a non-cleavable and membrane impermeable crosslinker. It contains an amine-reactive N-hydroxysuccinimide (NHS ester) and a sulfhydryl-reactive maleimide group. NHS esters react with primary amines at pH 7-9 to form stable amide bonds. Maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. The maleimide groups of Sulfo-SMCC and SMCC are unusually stable up to pH 7.5 because of the cyclohexane bridge in the spacer arm. Because it contains the hydrophilic sulfonyl moiety, Sulfo-SMCC is soluble up to ∼10 mM in water and many commonly used buffers, thus avoiding the use of organic solvents which may perturb protein structure.
Caution: For Research Use Only. Not for use in diagnostic procedures.
Specification Test Results
| Product Line | Pierce™ |
| Labeling Method | Chemical Labeling |
| Crosslinker Type | Heterobifunctional |
| Reactive Moiety | Maleimide, Sulfo-NHS Ester |
| Spacer | Short |
| Form | Powder |
| Quantity | 50 mg |
| Type | Crosslinker |
| Solubility | Water |
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